Calorimetric titrations for Nupr1, MSL1 and chemically-damaged-DNA interactions.
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Binding isotherms (normalized injection heat versus molar ratio of the reactants in the cell) are shown: (A) Nupr1 interacting with etoposide-damaged DNA. (B) MSL1 interacting with etoposide-damaged-DNA. (C) MSL1 interacting with Nupr1. (D) MSL1 interacting with Nupr1 + etoposide-damaged-DNA complex. All the titrations were carried out at 25°C in 10 mM acetate buffer (pH 4.5). The binding experiments involving damaged DNA were analyzed considering an estimated molecular weight of 10000 kDa.
创建时间：
2016-02-23
