Supplemental Table S1 - S4

<strong>Supplemental Table S1: Comparison of cellular size, shape, and speed of PAECs cultured on 0.22 kPa, 4 kPa, 30 kPa and plastic with respect to those cultured on 1.25 kPa hydrogels.</strong> Spread area, circularity, and speed of PAECs cultured on collagen-coated hydrogel of shear modulus 1.25 kPa versus those cultured on collagen-coated hydrogels with a shear modulus of 0.22 kPa, 4 kPa, 30 kPa, and plastic. These data are the mean ± standard deviation from five different independent experiments. These data are shown in Fig. 3. <br> <strong>Supplemental Table S2:</strong> <strong>Comparison of the PMF intensity (i.e., standard deviation of pixel intensity over 5 consecutive frames) at intercellular regions with respect to the regions without cells.</strong> The median value of the standard deviation of pixel intensity across five successive frames (defined as PMF intensity) is compared with the same property obtained in no-cell regions (i.e., regions without cells) for PAEC cultures on hydrogels with a shear modulus of 1.25 kPa versus 30 kPa. Comparison is shown for extracellular Ca²⁺ concentration of 2 mM versus 0.1 μM. These data are the mean ± standard deviation from five different independent experiments. The data is shown in Fig. 4. <br> <strong>Supplemental Table S3:</strong> <strong>Comparison of baseline PMF intensity of (A) PAECs cultured on 30 kPa versus on 1.25 kPa hydrogels, and (B) PAECs cultured in 0.1 μM extracellular Ca</strong>^{<strong>2+</strong>} <strong>versus 2 mM extracellular Ca</strong>^{<strong>2+</strong>}<strong>.</strong> On the left is the comparison of PAECs on 1.25 kPa hydrogel versus those on 30 kPa hydrogel. On the right is the comparison of PAECs in normocalcemic (2 mM Ca²⁺) versus hypocalcemic (0.1 μM Ca²⁺) culture medium. These data are the mean ± standard deviation from five different independent experiments. These two tables present two different comparisons of the same four sets of experiments shown in Fig. 5 and 6. <br> <strong>Supplemental Table S4: Comparison of the range and duration of ΔPMF in PAECs cultured on 30 kPa versus 1.25 kPa hydrogels.</strong> The first column shows a comparison of the range of ΔPMF between PAECs cultured on 1.25 kPa hydrogels versus those cultured on 30 kPa hydrogels. The second column shows a comparison of the duration of ΔPMF between PAECs cultured on 1.25 kPa hydrogels versus those cultured on 30 kPa hydrogels. These data are the mean ± standard deviation from five different independent experiments. These data are shown in Figs. 5 and 6.