m6A IP sequencing analysis of m6A mRNA methylation regulation by YTHDF2 in keratinocytes

N6-methyladenosine (m6A) RNA methylation is the most abundant internal chemical modifications in eukaryotic messenger RNA (mRNA) as well as long non-coding RNA (lncRNA). Recently, m6A RNA methylation research was revived by the discovery of the fat mass- and obesity-associated protein (FTO) as the first RNA demethylase, implicating that m6A RNA methylation is a reversible and dynamic modification and may have critical biological functions. Emerging evidence has shown that m6A modifications in mRNAs and lncRNAs play crucial roles in regulating RNA fate and function in biological processes in the past several years. The goal is to determine the targets of YTHDF2, a reader for m6A RNA methylation, in keratinocytes. m6A IP seq of control and YTHDF2-knockdown HaCaT keratinocytes