BCG_CNRL_wk0vs3_infantMM

To assess changes in immune gene expression following BCG vaccination in infants, 6 infant rhesus macaques were vaccinated with BCG at 2 weeks of age. Whole blood was collected at the time of BCG vaccination, and at 3 weeks post-BCG, in EDTA coated tubes. Peripheral blood mononuclear cells were collected by ficoll and RNA was extracted using Macherey Nagel nucleospin columns. RNA from both timepoints was then used for gene expression analysis using the Nanostring nCounter platform. Data from 12 infants was collected at pre-BCG and 3 weeks post-BCG timepoints using the NHP_Immunology_V2.0 770 gene panel. Differential gene expression in was run to measure changes from 0 to 3 weeks post-BCG vaccination.