TABLE 4 in DNA Barcoding of west European Strachiini questions the validity of several species of the genus Eurydema (Hemiptera. Heteroptera: Pentatomidae)
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TABLE 4. List of primers used for the protocol « two step NGS ». Primer NamePrimer Sequence 3’-5’PublicationCycle of amplificationFragment FCLCO1490TTTCAACWAATCATAAAGATATTGGFolmer et al. (1994)35XIll_C_RGGIGGRTAIACIGTTCAICCShokralla et al. (2015)94°C 40sec, 48°C 60sec, 72°C 30secFragment BRIll_B_FCCIGAYATRGCITTYCCICGShokralla et al. (2015)35XHCO2198TAAACTTCAGGGTGACCAAAAAATCAFolmer et al. (1994)94°C 40sec, 46°C 60sec, 72°C 30sec
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2025-12-22
