Lung gene expression profiling during acute lung injury and its modulation by IL-17A and IFN-gamma

HLA-DR3, HLA-DR3.IL17A knockout and HLA-DR3.IFN-gamma knockout (KO) mice were challenged intraperitoneally with 10 microgram of staphylococcal enterotoxin B. 4 and 24 hours later mice were euthanized, lungs harvested, RNA extracted and processed for gene expression analysis using Clariom S arrays in comparison to respective naive mice After transcardial perfusion of mice with 10 ml of PBS, lungs were removed, Total RNA was extracted from perfused lungs at 4- and 24- hours after SEB challenge. Gene expression profiling was determined using mouse Clariom S arrays at ThermoFisher Corporation in comparison to respective naive mice. Acute lung injury was induced in eight- to sixteen-week-old male and female mice by intraperitoneal injection of 10 micrograms of endotoxin-reduced staphylococcal enterotoxin B (SEB) (Toxin Technologies, Sarasota, FL) in PBS.