Tourmaline files for Sterivex eDNA testing

Tourmaline and QIIME 2 files for 12S (fish) and 16S (microbes) amplicon metabarcoding associated with Sterivex eDNA methods development in Biscayne Bay, Florida (https://github.com/aomlomics/sterivex).

Files are associated with sequence processing using either stand-alone QIIME 2 (12S folder) or Tourmaline (16S folder), which is a recently developed program that wraps QIIME 2 and Snakemake. Taxonomy was assigned to sequences using QIIME 2-formatted reference database for 16S (SILVA; v.138.1), 16S chloroplasts (PR2; v.4.12), and 12S (Mitohelper; March 2021 release).

Folders with intermediate and final output files:

16S folder - file outputs from Tourmaline

16S chloroplast folder - files from QIIME 2

12S folder - files from QIIME 2

Reference database artifact files (.qza) used to assign taxonomy:

silva-138-99-tax.qza

silva-138-99-seqs.qza

pr2_plastid_seqs.qza

pr2_plastid_tax.qza

12S-16S-18S-seqs_March2021.qza 

12S-16S-18S-tax_March2021.qza 

These files are associated with the following project: https://github.com/aomlomics/sterivex. Raw FASTQ sequence data are available in NCBI SRA under BioProject ID PRJNA728349.