Ascaris intestine calcium fluorescence dataset showing synergism of levamisole and Cry5B

A novel class of biocidal compounds are the Crystal 3D (Cry) and Cytolytic (Cyt) proteins produced by Bacillus thuringiensis (Bt). Some Bt Cry proteins have a selective nematocidal activity, with Cry5B being the most studied. Cry5B kills nematode parasites by binding selectively to membrane glycosphingolipids, then forming pores in the cell membranes of the intestine leading to damage. Cry5B selectively targets multiple species of nematodes from different clades and has no effect against mammalian hosts. Levamisole is a cholinomimetic anthelmintic that acts by selectively opening L-subtype nicotinic acetylcholine receptor ion-channels (L-AChRs) that have been found on muscles of nematodes.  A synergistic nematocidal interaction between levamisole and Cry5B has been described previously on whole worms, but the location, mechanism and time-course of this synergism is not known. In this study we follow the timeline of the effects of levamisole and Cry5B on the Ca2+ levels of enterocyte cel..., Measurement of Ca2­ fluorescence
All recordings were performed with a Nikon Eclipse TE300 microscope (20X/0.45 Nikon PlanFluor objective), fitted with a Photometrics Retiga R1 camera (Photometrics, Surrey, BC, Canada). Light control was achieved using a Lambda 10-2 two filter wheel system with a shutter controller (Lambda Instruments, Switzerland). Filter wheel one was set on a green filter (510-560 nM bandpass, Nikon USA) between the microscope and camera. Filter wheel two set on the blue filter (460-500 nM, bandpass, Nikon, USA) between a Lambda LS Xenon bulb light box which delivered light via a fiber optic cable to the microscope (Lambda Instruments, Switzerland). The blue light emission was controlled using a shutter. All Ca2+ signal recordings were acquired and analyzed using MetaFluor 7.10.2 (MDS Analytical Technologies, Sunnyvale, CA). Exposure times were 150ms with 2x binning.  Maximal Ca2+ signal amplitudes (ΔF/F0 %) for all stimuli applied were calculated using the equation ..., , # Williams et al: 2024 Ascaris suum intestine data sets for figures

[https://doi.org/10.5061/dryad.b5mkkwhkk](https://doi.org/10.5061/dryad.b5mkkwhkk)

We list the the raw observations in .xlsx format (Excel Workbook) that was entered into Prizm 5 files for the construction of Figures, 2C, 3B, 4D, 4E, 4F, 8D, 8E, 8F, 9B, 9C and 9D for the Figs 2C, 3B, 4D, 4E, 4F, 8D, 8E, 8F, 9B, 9C and 9D of the Plos Pathogens manuscript.

## Description of the data and file structure

The data files refer to each of the Figures for the manuscript prepared for Plos Pathogens. The data are listed in columns for each of the different treatments.

**Cry5B message targets are present in *A. suum* intestine**

Fig 2C:  Cry5B message targets are present in *A. suum* intestine. Datasets of the differential expression of Cry5B targets of Asu-bre-5 and *Asu-cdh-8)* between intestine and body wall. Bar chart (expressed as mean ±SEM) demonstrating transcript level analysis from intestinal samples for *bre-5* (gre...