Neutralization of BoNT by single mAbs in combination with FP.

Antibodies and FP:mAb complexes were tested for their ability to protect mice from lethality induced by botulinum neurotoxin (BoNT). mAbs were tested alone, without modification, or biotinylated and in combination with the fusion protein (FP) by mixing with the toxin in vitro and intravenous injection one hour later. Mice were observed for 5 days. The amounts of mAb and FP used per mouse (µg), the serotype of each BoNT (A or B), and the percent of surviving mice for each dose (LD50) administered are shown. Blank spaces indicate dose levels that were not tested.