S9 Data -

S6 Fig. (A) Specific glucose consumption rates were determined using rS = YXS • k, where rS is the specific glucose consumption rate, YXS is glucose consumed per unit biomass, and k is the exponential growth rate [55,56]. YXS was determined from the difference between glucose measurements between inoculation and late exponential/early stationary phase. Error bars = SD; n = 3. Different letters indicate statistically different values (p < 0.05) as determined by ordinary one-way ANOVA with Tukey’s multiple comparison. (B) Glucose consumed = (Glucose t0—Glucose tx) / (Glucose t0) × 100%, where t0 = the time of inoculation and tx = time after culture growth had ceased. All glucose measurements at tx were below the limit of detection. (C) Replicates of competition experiments with wild-type and luxO D61E strains. Wild-type (marked with Tm) and luxO D61E (marked with Spec) strains of DS40M4 were inoculated at initial frequencies of 80%, 50%, or 20% wild-type (WT) in M9G medium and grown to near stationary phase at t = 16 h, and then diluted to maintain growth in log-phase. Colony forming units (cfus/mL) were measured at each time point using selective antibiotics for each strain. The total population number was calculated, and the data are represented as the percent of WT cells in the total population of WT and luxO D61E.
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