Brassica napus reduced representative library sequencing

We developed a technique called restriction fragment associated polymorphism (RFAP) by integrating the AFLP and Illumina sequencing technology. To illustrate our modeling approach, a common set of restriction fragments across a double haploid (DH) population (BnaNZDH) of highly established allotetraploid Brassica napus and its two parents (ZY821 and No2127)were sequenced. Allelic SNPs and the presence/absence variations (PAVs) were identified using this pipeline. Two parallel linkage maps, one SNP bin map containing 8780 SNP loci and one PAV linkage map containing 12,423 dominant loci, were constructed.