TABLE 1 in Morphology and multigene phylogeny reveal a novel Stagonospora species (Massarinaceae, Dothideomycetes) from Thailand

TABLE 1. The amplified loci, primers, and PCR thermal cycle protocols were used in this study.
Genes/lociPCR primers (forward/reverse) PCR conditionsReferenceLSULR0R/LR595 °C; 5 min (94 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 minVilgalys & Hester (1990)ITS SSUITS1 / ITS4 NS1 / NS494 °C; 2 min (95 °C; 30 s, 55 °C; 50 s, 72 °C; 90 s) × 35 thermal cycles, 72 °C; 10 minWhite et al. (1990)tef 1-αEF1-983F / EF1-2218R95 °C; 5 min (95 °C; 30 s, 58 °C; 50 s, 72 °C; 1 min) Rehner & Buckley (2005) × 35 thermal cycles, 72 °C; 10 min