Evidence for a degenerated Y chromosome in Micropoecilia bifurca.

Microsatellite markers were selected using the guppy (Poecilia reticulata) female or male assembly to find markers in positions spanning the whole chromosome. Three markers showed no variants in the female sample. The other six "informative" markers had two or more different alleles in females (and both homozygotes and heterozygotes were seen), but they all appear to be hemizygous in males (different alleles were found in the males also, but all males that could be genotyped appeared to be homozygotes; at least 13 males support this conclusion for each informative marker). The identities of the M. bifurca samples studied were verified using cox1 sequences, as for M. picta and M. parae (see S5 Table). (XLSX)