RNA-seq of endothelial cells, astrocytes and microglia in cell-type specific Nrf2 knockout mice, in a model of system inflammation

Nrf2 is a potential therapeutic target for various neurological disorders including neurodegenerative diseases, but the mechanisms behind Nrf2-mediated cytoprotection are incompletely understood. While an anti-inflammatory effect is a popular hypothesis, Nrf2 is highly expressed in microglia, astrocytes and brain endothelial cells in the adult brain, and the brain cell type-specific role of Nrf2 in regulating the basal transcriptome and controlling neuroinflammation is unknown. To address this, we employed three inducible conditional Nrf2 knockout mice in which Nrf2 is deleted in microglia, astrocytes, and brain endothelial cells respectively, in a model of system inflammatory challenge.