Sulfur assimilation improves SAM-dependent methylation

We employed omic data and conducted comparative analysis in Saccharomyces cerevisiae strains with different S-adenosine-L-methionine (SAM) level. This strategy can help us locate the limiting factor for SAM availability. We found sulfur assimilation emerged as the most significantly enriched pathway and further confirmed that sulfur assimilation was the dominant limiting step of SAM biosynthesis in the whole cell system. Overall design: Transcriptome analysis of Saccharomyces cerevisiae strains with different SAM levels