Diisononyl phthalate exposure inducing adipocyte differentiation

Diisononyl phthalate (DINP) is a high molecular weight phthalate and is considered to be an environmentally friendly phthalate, with significantly lower carcinogenicity and reproductive toxicity compared to DEHP and DBP. DINP has been recently introduced as DEHP substitute and commonly used in a large variety of plastic items, especially as plasticizer in PVC products. Consequently, DINP has now become one of the major phthalate compounds in the indoor and outdoor environmental media. Fat tissue is a main target tissue for DINP. we determined the effects of DINP on adipogenesis in 3T3-L1 preadipocytes, explored the modes of action and analyzed the systematic toxicities through global transcriptomic response to DINP. The results showed that DINP could induce adipogenesis by PPAR? pathway, and the global gene expression analysis revealed that promoting differentiation and lipid metabolism are the primary effects for DINP exposure on preadipocytes.