Real-time quantitative PCR analysis for validation of miRNAs expressed during osteogenic differentiation of bone marrow MSCs versus healthy donors MSCs. Homo sapiens

Total small RNA was isolated from BM-MSCs obtained from OA diagnosed patients (n=10) and non-OA (n=10 after osteogenic induction for 1, 10 and 21 days. miRNAs, were initially screened and those with consistent differential expression were selected for further validation by qRT-PCR analysis. Overall design: From an initial screening of 246 differentially expressed miRNAs (fold change ≥± 2, P ≤0.05) between OA and non-OA BM-MSC samples 21 were selected and validated by qRT-PCR