RNA-seq analysis of Calreticulin(Calr) knockout LLC cells identified ER stress response

To investigate the mechanisms by which Calreticulin inactivation increases tumor sensitivity to T cell-mediated anti-tumor immunity, LLC cells were transduced with sgRNA targeting Calreticulin or a control sgRNA, and transcriptome changes were profiled using paired-end RNA-seq. Our data demonstrated that Calr KO induces an ER stress response. LLC cells were transiently transduced with plasmids expressing Cas9 and sgRNA targeting Calreticulin or a control sgRNA, and cultured in vitro for 10 days to achieve knockout efficiency. For each group, cells were seeded into 6-well plates in triplicate and harvested by TRIzol after 48 hours. Comparative gene expression profiling was then performed.