Schematic view of panel development for pulmonary IPA.

Schematic view of strategy for discovery, qualification, and verification of panel-based classifiers. The BAP fractionation platform fractionates proteins and peptides for analysis by automated size exclusion chromatography (SEC). Candidate biomarkers were assembled based on proteins identified in the discovery phase and by previous studies. For each candidate, targeted proteomics assays using stable isotope dilution (SID)-selected reaction monitoring (SRM) assays were developed, standardized, and used to quantitate the abundance of the candidate biomarker in the discovery population (qualification). Nonparametric statistical filters were used to identify 15 host response proteins/peptides and 2 fungal polysaccharides. SID-SRM-MS measurement of host-response proteins and peptides were used to test RF classifier performance.