RNA-Seq data of ruminal epithelial tissue and 16S rRNA sequencing data of rumen digesta in goats infusion of three short-chain fatty acids [16s rRNA-seq]

Emerging data has highlighted the importance of short-chain fatty acids (SCFAs), particularly butyrate, in regulating ruminal homeostasis in vivo isolated epithelial cells. However, little is known about other SCFAs like acetate or propionate, and the interaction between rumen microbes and epithelial immunity are rarely reported. Here, we firstly combined infusion of three SCFAs, to study their different roles in ruminal development, antioxidant capacity, barrier functions, and immunity, as well as cross-talk with ruminal microbiome (16S rRNA sequencing data of rumen digesta) and derived transcriptome (RNA-Seq) and metabolism using an in vivo goat model. Overall design: A total of twenty-four healthy multiparous Guanzhong goats with an average body weight of 47.44 ± 3.38 kg at 1.5 years old in latter half of gestation period were selected in this study. All dairy goats were then randomly divided into four groups (n = 6 for each group) after adaption with the diet and light conditions to receive different SCFAs infusion treatments. They were assigned to a normal control group infusion with normal saline (NC), an infusion with sodium acetate solution (SA), an infusion with sodium propionate solution (SP), and an infusion with sodium butyrate solution (SB) according to previous studies in ruminants. The same volume of SCFAs and normal saline solutions at 1 L were slowly perfused into the rumen with a rumen tube daily before morning feeding. All solutions were calibrated to be consistent with a pH meter before infusion. The experiment lasted for 13 days, which combined with a 12-days of infusion period and a 1-day of sampling period. All goats were fasted for 12 hours before the sampling day. The animals were then weighed, and slaughtered and dressed by professional butchers. Firstly, the rumen digesta was immediately collected and stored directly in liquid nitrogen for the analysis of rumen microbiome using 16S rRNA sequencing. Then, another part of 5 g rumen epithelial tissue was rapidly frozen in liquid nitrogen for rumen epithelial RNA-Seq.