Global genomic profiling of estrogen-regulated genes in bone marrow B cells under hypoxia

In women, estrogen deficiency after menopause frequently accelerates osteoclastic bone resorption, leading to osteoporosis, the most common skeletal disorder. However, mechanisms underlying osteoporosis resulting from estrogen deficiency remain largely unknown. To gain new insights into the impact of estrogen on bone marrow B cells, we used high-throughput sequencing to analyze global estrogen transcriptional networks in WT bone marrow B cells under hypoxic culture. Overall design: RNA-seq was used to define estrogen regulated genes in bone marrow B cells from WT mice.