The specificity of exoCasMINI for gene editing.[Tag-seq]

The exoCasMINI exhibits similar specificity for gene editing. Compared to SpCas9, exoCasMINI also exhibited higher specificity for genomic DNA cleavage at the tested sites. Overall design: We evaluated the specificity of exoCasMINI-mediated gene editing in human cells using Tag-seq. Within the 10 tested sites, exoCasMINI exhibited about 2.5-fold increased activity and similar specificity compared to CasMINI. we also compared the DNA cleavage activity of SpCas9, LbCas12a, CasMINI, and exoCasMINI across four genomic sites at the same location and five sites at adjacent locations, exoCasMINI also exhibited higher specificity for genomic DNA cleavage at the tested sites.