Jurkat CRISPRi ATAC-seq [Series 3]

We develop a system for bidirectional epigenetic editing (CRISPRai), in which orthogonal activating (CRISPRa) and repressive (CRISPRi) perturbations are applied simultaneously to multiple loci the same cell. We perform ATAC-seq on CRISPRi perturbed Jurkat T cells to investigate chromatin accessibility changes upon perturbation of individual regulatory elements. Overall design: ATAC-seq of CRISPRi perturbed Jurkat T cells E2 - Jurkat E2 CRISPRi ATAC-seq E4 - Jurkat E4 CRISPRi ATAC-seq E6 - Jurkat E6 CRISPRi ATAC-seq E9 - Jurkat E9 CRISPRi ATAC-seq NTC - Jurkat NTC CRISPRi ATAC-seq TSS - Jurkat TSS CRISPRi ATAC-seq