Quantitative SAM analysis using a continuum of age versus gene expression produces network hubs that are inhibited with human muscle age.

Using a total of 97 U133+2 Affymetrix gene-chips newly produced from two independent studies, the DRET study and the HERITAGE family study we produced a novel analysis that relied on the full age-range present in these data sets. A) We first found a set of genes that co-varied with age in the DRET study and then confirmed that 580 of these were also related to age in the HERITAGE study. Mitochondrial genes were not a feature of this linear age vs gene analysis. We then mapped the Affymetrix probe-sets to the IPA database and examined the up-stream analysis output. We found in IPA that the age-related dataset was consistent with including c-MYC (z-score = −2.8 and p-value