Hevea brasiliensis subsp. brasiliensis strain:clone RRIM 600 Transcriptome or Gene expression. Hevea brasiliensis subsp. brasiliensis strain:clone RRIM 600

Hevea brasiliensis is the major economic crop as a source of natural rubber production. However, limited information of genomic resource and the lack of molecular markers developed for this plant species hinder breeding programs for crop improvement. To gain a better understanding of the rubber tree genome, we generated Expressed Sequence Tags (ESTs) and developed microsatellite markers from the transcriptome of H. brasiliensis leaf. We have sequenced 2,265,782 reads from the using 454 pyrosequencing technology. After clustering and assembly, a total of 214,013 unique sequences, comprising 32,633 contigs and 181,380 singlets, were generated. Gene annotation revealed that 49% shared sequence similarity with proteins in the Genbank non-redundant protein database. Functional categorization was performed by mapping against Gene Ontology (GO). We also identified 43,865 loci microsatellites from the data set.