Distinct roles of LARP1 and 4EBP1/2 in regulating translation and stability of 5′TOP mRNAs [LARP1]

To investigate the changes in gene expression upon loss of LARP1, we performed RNA-seq and differential gene expression analysis on WT and derived LARP1 KO cell lines. The canonical and 5′TOP mRNA cell lines used for single-molecule imaging were treated as biological replicates for the differential gene expression analysis. WT cells (canonical and 5′TOP mRNA cell lines) and derived LARP1 KO clones (4 for each cell line, 8 clones total) were analyzed by RNA-seq, using independent replicates (cells harvested on different days).