Recombinant PNGaseF production and deglycosylation assays
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https://zenodo.org/record/3263901
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资源简介：
Recombinant PNGaseF production and deglycosylation assays. Open Lab notebook.
1.5 L of BL21 E.coli cells harbouring the pOPH6:EmPNGaseF vector encoding a His-tagged construct of Elizabethkingia miricola PNGase F (EmPNGaseF, Uniprot P21163, PNGF_ELIMR) were used to express the protein. EmPNGAse F was purified from the E.coli lysate by immobilised metal affinity and size exclusion chromatography and its activity assayed against 5 distinct glycoproteins.
创建时间：
2024-07-22
