Diamond2GO Database – Version 2025-08-12 (nr_clean_d2go)

This is the updated Diamond2GO reference database built on 12th August 2025. It is a DIAMOND-formatted protein database (`.dmnd`) consisting of over 27 million sequences derived from the NCBI `nr` dataset, filtered to include only those with Gene Ontology (GO) annotations, and redundancy reduction using MMseqs2 (95% similarity). This version improves sensitivity and annotation coverage compared to the original 2023 release used in the published D2GO manuscript, and the earlier 2025 release. This database is intended for use with the Diamond2GO tool, which enables rapid GO-term annotation and enrichment analysis for high-throughput sequencing datasets. For reproducibility of results published using the earlier version (699,409 sequences), please refer to the [v1.0.0 release] https://github.com/rhysf/Diamond2GO/releases/tag/6a035ce