Bacterial Profiling of Colorectal Cancer Biopsies: A Culture-Based Study in Indian Patients

The role of microorganisms that initiate and facilitate the process of colorectal cancer has become clear now, as cited in various research reports. More information is needed about mechanisms through which the microbial community or single bacterium promotes carcinogenesis. The present work deals with the isolation and identification of bacteria from 9 colorectal cancer biopsy samples and 9 adjacent biopsy samples. Different media, such as Brain heart infusion (BHI), Anaerobic Basal Agar (ABA), and Trypticase soy agar (TSA), and culture conditions have been manipulated to maximize the tapping of bacteria residing in biopsy samples. A total of 75 bacteria were isolated from the tumorus and adjacent site. Enterococcus, Escherichia, Klebsiella, Shigella, Citrobactror, Morganella and Veillonella have been found to be enriched in most of the cancerous biopsies, while biopsies collected from adjacent tissues had Escherichia,Shigella, Enterococcus, and Streptococcus bacteria. A culture-based approach to determining bacterial diversity has its own advantage, which allows the study of a single bacterium to understand the events leading to the development of intestinal carcinogenesis. This culture-based approach may provide novel insight into the pathology and potentially lead to new therapeutic modalities targeting the specific bacteria implicated in the inflammation and carcinogenesis of colorectal cancer.

近年来，关于启动并促进结直肠癌过程的微生物的作用已日渐明朗，如众多研究报告所提及。关于微生物群落或单个细菌如何促进癌变机制的详细信息仍需进一步研究。本研究涉及从9个结直肠癌活检样本和9个邻近活检样本中分离和鉴定细菌。通过操控不同的培养基，如脑心浸液（BHI）、厌氧基础琼脂（ABA）和胰蛋白酶大豆琼脂（TSA），以及培养条件，以期最大限度地提取活检样本中驻留的细菌。共从肿瘤及其邻近部位分离出75种细菌。在大多数癌性活检样本中发现肠球菌、大肠杆菌、克雷伯菌、志贺菌、奇异菌、摩根菌和韦荣菌富集，而邻近组织收集的活检样本则含有大肠杆菌、志贺菌、肠球菌和链球菌。基于培养的方法确定细菌多样性具有自身优势，它允许通过研究单个细菌来理解导致肠道癌变发展的事件。这种基于培养的方法可能为病理学提供新的见解，并可能引领针对结直肠癌炎症和癌变中涉及的特定细菌的新治疗方法。