Genes expression profile in BMDM challenging with S1P or DAMPs

To explore the differently expressed genes in BMDM after the treatment with S1P or DAMPs (Damage associated molecular patterns). BMDM cells were incubated with apoptotic cell culture medium (ACM) or S1P (500nM) for 24 hours before harvest for RNA extraction. ACM generated from palmitic acid treated AML12 cells was used as DAMPs. We also want to see whether these genes regulated by S1P or DAMPs are Trem2 dependent or independent. Overall design: Three different treatments, DAMPs (ACM, apoptotic cell culture medium), S1P, and untreated control, were applied to primary BMDMs from mice of two different genotypes: Trem2(-/-) and WT. Each treatment/genotype combination was executed as a replicate of 4.