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METHODS and COMPOSITIONS FOR TOLERANCE TO THE GLYPHOSATE IN PLANTS

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present are directed to methods and compositions for conferring tolerance to the glyphosate of plants. Also provides plants, seeds, plant tissues, cells and portions of facilities to the glyphosate tolerant that comprise modified EPSP and of recombinant dna molecules which encode EPSP proteins, as well as methods for producing the same and their use. Claim 1: One molecule of recombinant dna which encodes EPSPS tolerant to glyphosate, where EPSPS comprising less one to the first replacement Of amino acids which are selected from the group which consists in: I6P-one, I6W-one, T17M-one, N28A-one, N28C-one, N28G-one, N28H-one, N28M-one, N28Q-one, N28S-one, N28T-one, N28V-one, L33E-one, A35M-one, L36E-one, E38F-one, G39k, G39W, T41H-one, V43P-one, V43Q-one, N45G-one, L46C-one, L46D-one, L46W-one, E50F-one, Y54G-one, L56E-one, L56K-one, A58I-one, R60E-one, R60K-one, R60Q-one, T61E-one, L62F-one, G63L-one, L64G-one, S65K-one, S65Q-one, S65R-one, E67C-one, K70l, K70W, A71M-one, K73P-one, V77N-one, G82Q-one, V86C-one, G101A-one, G101E-one, T102F-one, T102G-one, T102I-one, T102L-one, T102Q-one, T102V-one, A103C-one, A103D-one, A103F-one, A103G-one, A103I-one, A103L-one, A103P-one, A103R-one, A103v, R105A, P106A-one, P106C-one, P106G-one, P106I-one, P106L-one, P106Q-one, P106S-one, P106T-one, P106V-one, P106W-one, L107A-one, L107C-one, L107F-one, L107G-one, L107K-one, L107M-one, L107Q-one, L107S-one, L107T-one, L107v, L107W, V111N-one, V111Q-one, T112V-one, T112W-one, A114C-one, A114K-one, G115S-one, A118F-one, L122D-one, G124K-one, V125D-one, E130R-one, P132D-one, I133M-one, G144D-one, V160P-one, N161W-one, K170V-one, S179I-one, P190L-one, L191d, A192T, G194Q-one, K203A-one, R219F-one, T269C-one, T278N-one, L280D-one, L280R-one, E288I-one, A295F-one, V297Q-one, T307W-one, G315K-one, M326A-one, K328F-one, D331M-one, V332K-one, V332Q-one, A333I-one, A340Y-one, R35OK-one, E378L-one, E378W-one, E379m, E379N, Y383E, P418G, and C426M, where the position of substitution of the amino acid is from the position of the amino acid in the sequence provided as SEQ ID no: 1. Claim 2: The molecule of recombinant dna of the claim 1, where the EPSPS tolerant to the glyphosate is one of EPSPS to the glyphosate tolerant corn.
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国家林业和草原科学数据中心
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2023-02-12
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