Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells. Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to ambient temperature change is predicted to involve complex regulation. To assess the effects of temperature change within the readily tolerated temperature range on the transcriptome, we have performed expression profiling with S2R+ cells, which are derived from the ectothermic organism Drosophila melanogaster. Overall design: Aliquots of S2R+ cells were plated and shifted to different temperatures (11, 14, 25, and 30°C). Twenty-four hours after the temperature shift, total RNA was isolated and processed for hybridization of microarrays. Three replicate experiments were preformed.