Oligomerization of Cry1Ac in the presence of BBMV from resistant and susceptible larvae of P. gossypiella.

Cry1Ac activated toxin (0.5 μg) was incubated with BBMV (15 μg) from P. gossypiella susceptible APHIS-S (lane 5), resistant AZP-R (lane 6) separated by SDS-PAGE after three min heating at 50°C and revealed in western blot using anti-Cry1Ac antibody. Lane 1, shows MW markers; lane 2, Cry1Ac activated toxin; lane 3, BBMV from APHIS-S; lane 4, BBMV from AZP-R. Numbers under the 200 kDa bands represent the percentage of pixels relative to the 200 kDa band of APHIS-S BBMV with Cry1Ac toxin that corresponds to 100, calculated after scanning densitometry of the bands.