scRNA-seq analysis of Graves disease and healthy thyroid glands

We used single-cell transcriptomics to compare thyroid follicular cells (TFCs) and stromal cells from control and Graves' disease patients and gain insight into the contribution of non-immune cells to the autoimmune response of the thyroid gland.Enzimatically dispersed TFCs and stromal thyroid cells preparations were further enriched by magnetic depletion of infiltrating CD45+ leukocytes using RosetteSep Human CD45 Depletion Cocktail.本研究采用单细胞转录组学（single-cell transcriptomics）技术，对比对照组与格雷夫斯病（Graves' disease）患者的甲状腺滤泡细胞（thyroid follicular cells, TFCs）及甲状腺基质细胞，以深入解析非免疫细胞对甲状腺自身免疫应答的贡献。经酶解法分散的甲状腺滤泡细胞与甲状腺基质细胞悬液，进一步通过RosetteSep Human CD45 Depletion Cocktail磁珠耗竭试剂，利用磁耗竭法去除浸润性CD45+白细胞（CD45+ leukocytes），实现目标细胞的富集。

搜集汇总

背景与挑战

背景概述

该数据集包含9个单细胞RNA测序（scRNA-seq）实验，专注于人类Graves病和健康甲状腺的对比分析，涵盖5个女性Graves病样本和4个对照样本（包括男性和女性）。所有数据均使用Illumina NovaSeq 6000平台生成，以fastq格式提供，总数据规模达数十亿碱基，适用于甲状腺疾病的分子机制研究。