Effect of VIRMA amplification on gene expression in breast cancer cells

To confirm the contribution of elevated VIRMA levels towards cell proliferation and colony formation, we depleted VIRMA in four breast cancer cell lines, SKBR3, HS578T, MCF7 and MDAMB453 using short-hairpin RNAs (shRNAs). We then performed gene expression profiling analysis using data obtained from RNA-seq data Overall design: Comparative gene expression profiling analysis of RNA-seq data for VIRMA-overexpressing SKBR3 cells and eGFP control