RNA sequencing of three dimensional endothelial spheroids seeded in an artificial collagen matrix with or without VEGF stimulation

This study investigates the influence of VEGF on the transcriptome of Human umbilical vein endothelial cells (HUVECs) formed to a spheroid and seeded in a 3D collagen matrix in a standard spheroid sprouting assay as one of the most commen angiogenesis assay. Comparative gene expression profiling analysis of RNA-seq data for HUVEC spheroids from a spheroid sprouting assay with n=5 VEGF stimulated samples and n=5 spheroids treated with just basel medium