Data Sheet S2 – 16S rRNA gene sequence counts and taxonomy from CROMO, California USA CSW1.1 well depth profile May 2016

Data included are final 16S rRNA gene sequence counts and taxonomy information from five depths in the CSW1.1 well at the Coast Range Ophiolite Microbial Observatory, California, USA collected during a one-day sampling event in May 2016. Water samples for DNA extraction were collected from each depth using a peristaltic pump and sterile tubing. A 0.2 µm Sterivex filter was attached to collect cells, and DNA was extracted using phenol/chloroform extractions and precipitated with ethanol before purification using a Genomic Clean and Concentrate Kit (Zymo Research) and quantification via Qubit dsDNA High Sensitivity Assay. Purified bacterial samples were sequenced at the Genomics Core Facility at Michigan State University for analysis and processed on an Illumina MiSeq instrument. Sequences were processed and taxonomy was assigned using the SILVA SSURef alignment (v132) in Mothur. Filtering of common contaminants identified in Census of Deep Life samples was performed as described previously by Sheik et al., 2018. Data was collected to identify the organisms present at each depth in the profile.