Unravelling the impact of aging on the human endothelial lncRNA transcriptome [HUVEC CTL_vs_PCAT14 RNA-seq]

Gene expression profiling analysis was performed using the RNA-Seq data obtained from endothelial cells expressing or knockdown for PCAT14 Overall design: Human umbilical vein endothelial cells were transfected with 50nmol/L GapmeR targerting PCAT14 or the respective negative control (CTL). After 48h of incubation RNA was harvested with RNeasy mini plus kit (Qiagen) and whole transcriptome RNA sequencing analysis was performed.