[E-MTAB-308] Zebrafish gene 3 prime end pull down for genome annotation. Danio rerio

As part of the zebrafish genome annotation project the 3 prime ends of genes were pulled down on polyT beads and sequenced on the Illumina Genome Analyzer to identify alternative 3 prime ends in a range of tissues and developmental stages. Total RNA from a range of developmental stages and adult tissues were chemically fragmented, pulled down on polyT magnetic beads and double stranded cDNA was synthesized. The cDNA was BpmI digested to release from the beads and to leave a 6 T base tail. Illumina sequencing libraries were made followed by 76 base paired-end sequencing. ArrayExpress Release Date: 2010-10-14 Person Roles: submitter Person Last Name: Collins Person First Name: John Person Mid Initials: E Person Email: [e-mail] Person Phone: [phone] Person Address: Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1HH, United Kingdom Person Affiliation: Wellcome Trust Sanger Institute Overall design: Experimental Design: development_or_differentiation_design AEExperimentType: transcription profiling by high throughput sequencing Experimental Factor Name: AGE Experimental Factor Name: ORGANISM_PART Experimental Factor Name: DEVELOPMENTAL_STAGE Experimental Factor Name: SEX Experimental Factor Type: age Experimental Factor Type: organism_part Experimental Factor Type: developmental_stage Experimental Factor Type: sex