Age-dependent changes in transcription factor FOXO targeting in Drosophila melanogaster

FOXO transcription factors control numerous pathways involving metabolism, stress response, and longevity. Although direct targets of FOXO have been reported in various long-lived mutants and under stress conditions, no studies have investigated how normal aging impacts the cellular activity of FOXO. In this study, we compared genome wide dFOXO-bound sites in young and aged wild-type flies kept under normal feeding conditions to evaluate the dynamics of FOXO gene targeting during aging. Our results provide a new insight into FOXO chromatin targeting under a normal aging model and highlight the diverse regulatory mechanisms for FOXO transcriptional activity that are currently less understood. Overall design: ChIP DNA was prepared from 2-week and 5-week yw;+;+ flies. Two biological replicates were collected for each sample using dFOXO antibody. ChIP-seq libraries were prepared using the standard protocol with indexed adaptors and pooled for sequencing.