Single-cell transcriptomic profiling of mouse enteric neurons and enteric mesenchymal cells in enteric neurosphere cultures.

This study was undertaken to define the molecular subtypes of cells within enteric neurospheres. Methods: We performed single-cell RNA sequencing on 3-dimensional neurosphere cultures derived from the small intestines of Plp1::GFP mice. These data show profiling results from the PLP1::GFP negative cell fraction ancillary to the positive fraction in series GSE184981. Results: We identify populations of enteric neurons and fibroblasts in the PLP1::GFP negative cell fraction of enteric neurospheres. Use of 10X Genomics technology to analyze single-cell transcriptomics in 3-dimensional neurosphere cultures derived from the small intestine after performing FACS to purify the PLP1::GFP negative cell fraction.