Skeletal Muscle and Peripheral Nerve Histopathology in COVID-19

To explore the spectrum of skeletal muscle and nerve pathology of patients
who died following SARS-CoV-2 infection and assess for direct viral
invasion of these tissues. Psoas muscle and femoral nerve were sampled
from 35 consecutive autopsies of patients who died following SARS-CoV-2
infection and 10 SARS-CoV-2-negative controls were examined under light
microscopy. Clinical and laboratory data were obtained by chart review.
 In SARS-CoV-2-positive patients, mean age at death was 67.8
years (range 43-90 years) and the duration of symptom onset to death
ranged from 1-49 days. Four patients had neuromuscular symptoms. Peak
creatine kinase was elevated in 74% (mean 959 U/L, range 29-8413 U/L).
Muscle showed type 2 atrophy in 32 patients, necrotizing myopathy in 9,
and myositis in 7. Neuritis was seen in 9. Major histocompatibility
complex-1 (MHC-1) expression was observed in all cases of necrotizing
myopathy and myositis and 8 additional patients. Abnormal expression of
myxovirus resistance protein A (MxA) was present on capillaries in muscle
in 9 patients and in nerve in 7. SARS-CoV-2 immunohistochemistry was
negative in muscle and nerve in all patients. In the 10 controls, muscle
showed type 2 atrophy in all patients, necrotic muscle fibers in 1, MHC-1
expression in non-necrotic/non-regenerating fibers in 3, MxA expression on
capillaries in 2, and inflammatory cells in none, and nerves showed no
inflammatory cells or MxA expression. Muscle and nerve tissue demonstrated
inflammatory/immune-mediated damage likely related to release of
cytokines. There was no evidence of direct SARS-CoV-2 invasion of these
tissues.