Single-cell RNA-sequencing of KPPF;Col1smaKO mouse models with pancreatic tumors

We employed dual-recombinase genetic mouse models of spontaneous PDAC mice (KPPF;Col1smaKO) to delete Col1 (type I collagen) specifically in myofibroblasts, in comparison with control KPPF mice. Single-cell RNA-sequencing analyses were performed on unfractionated live cell mixtures from pancreatic tumors of KPPF mice and KPPF;Col1smaKO mice, to investigate the impact of myofibroblast-specific Col1 deletion on tumor microenvironment. Overall design: Unfractionated live cell mixtures from pancreatic tumors of KPPF control mice and KPPF;Col1smaKO mice were analyzed by 10x single-cell RNA-sequencing.