Data, R scripts, and raw figure file for: "Polyploidy and range position affect the intensity and outcome of a specialized plant-pollinator interaction"

README: Description: These files contain all data text files, R scripts, and the Excel file with the raw version of the figures used to do all the statistical analyses and to prepare the raw version of the figures included in manuscript entitled “Polyploidy and range position affect the intensity and outcome of a specialized plant-pollinator interaction”. There are seven data files (file type: tab-delimited .txt), two R-script files (file type: .R), and one file with the raw versions of the figures (file type: .xlsx) with 20 sheets. Data files:1. FieldSurvey_AcrossPops.txt2. CapsuleData_AcrossPops.txt3. DWPOL_AcrossPops.txt4. FieldSurvey_MIN.txt5. CapsuleData_MIN.txt6. DWPOL_MIN.txt7. PopCoordinates.txt R-script files:8. Script_StatisticsForMS.R9. Script_AcrossPopMap.R File with the raw versions of the figures:10. Figures.xlsx Data files FieldSurvey_AcrossPops.txt: This tab-delimited text file contains fruit-set data (i.e. whether capsules 2 to 6 counted from the bottom of the inflorescence were developing, withering (empty), lost, or eaten by herbivores) from scorings in 35 populations of Litnophragma bolynderi. This data file, or a subset thereof, was used in the R-script file Script_StatisticsForMS.R and contains the following columns: ID: Individual number for each plant. POPid: Population-specific individual number for each plant. Population: Three-letter abbreviation of the population name. PloidyLevel: Ploidy level of the major cytotypes in the population. 2x: diploid population; 4x: tetraploid population; 6x: hexaploidy population; 2x4x: diploid, tetraploid mixed population; 4x6x: diploid, tetraploid mixed population; 2x4x6x: diploid, tetraploid, hexaploid mixed population; unknown: ploidy level of plants in this population is unknown. The information on the ploidy level was extracted from Gross et al. (2025) PNAS, 122(33): e2505119122, https://doi.org/10.1073/pnas.2505119122. Developing: Number of developing capsules, considering capsules 2 to 6 counted from the bottom of the inflorescence. Withering: Number of withering (empty) capsules, considering capsules 2 to 6 counted from the bottom of the inflorescence. Eaten: Number of capsules eaten by herbivores, considering capsules 2 to 6 counted from the bottom of the inflorescence. Malformed: Number of malformed capsules, considering capsules 2 to 6 counted from the bottom of the inflorescence. TooYoung: Number of capsules in a too early stage to be able to see whether they are developing or withering, considering capsules 2 to 6 counted from the bottom of the inflorescence. Missing: Number of capsules that were missing (e.g. broken off), considering capsules 2 to 6 counted from the bottom of the inflorescence. Nocap: Number of capsules that could not be considered (e.g. because there were fewer than 7 capsules produced by an individual), considering capsules 2 to 6 counted from the bottom of the inflorescence. PropDev: Proportion of developing capsules when only considering developing and withering capsules. This was calculated as Developing / (Developing + Withering). PropEat: Proportion of capsules eaten when considering all 5 capsules recorded. This was calculated as Eaten / 5. PropDevTOT: Proportion of developing capsules when considering all 5 capsules recorded. This was calculated as Developing / 5. nScored: Number of capsules scored. This was calculated as Developing + Withering + Eaten + Malformed + TooYoung + Missing. Lat: Latitude of the population in °N. Long: Longitude of the population in °E. Alt: Elevation of the population in m a.s.l. CapsuleData_AcrossPops.txt: This tab-delimited text file contains seed-set data and data on the Greya politella offspring of developing capsules collected in 26 populations of Litnophragma bolynderi. This data file, or a subset thereof, was used in the R-script file Script_StatisticsForMS.R and contains the following columns: Pop: Three-letter abbreviation of the population name. PlantNumber: Population-specific individual number for each plant. PlantID: Individual ID for each plant, which is a combination pf Pop and PlantNumber. POLTraces: Indication of whether the capsule contained traces (eggs, larvae, or eaten seeds) of Greya politella (Yes) or not (No). POL: Indication of the presence (1) or absence (0) of traces (eggs, larvae, or eaten seeds) of Greya politella. WholeSeeds: Number of intact developing ovules. UndevOvules: Number of undeveloped ovules. EatenSeeds: Number of partly eaten, developing ovules. Only eaten seeds with more than half the seed coat remaining were noted to avoid counting several smaller fragments originating from the same seed. N_Eggs: Number of Greya politella eggs. NA: No Greya politella eggs were present. N_Larvae: Number of Greya politella larvae. NA: No Greya politella larvae were present. N_Offspring: Number of Greya politella offspring. This was calculated as N_Eggs + N_Larvae. NA: No Greya politella offspring were present. OffspringStage: Indicating the stage of development of the Greya politella offspring in the capsule. Egg: only Greya politella eggs were present; Larvae: Greya politella larvae or Greya politella eggs and larvae were present; NA: no Greya politella offspring were present. N_EggsLarvae: Number of Greya politella eggs for capsules with only Greya politella eggs but no larvae and number of Greya politella larvae for capsules with Greya politella larvae or eggs and larvae. NA: no Greya politella offspring were present. DevOvules: Total number of developing ovules. This was calculated as Whole seeds + EatenSeeds. TotOvules: Total number of ovules. This was calculated as DevOvules + UndevOvules. PropDev: Proportion of developing ovules. This was calculated as DevOvules / TotOvules. Ploidy: Ploidy level of the major cytotypes in the population. 2x: diploid population; 4x: tetraploid population; 6x: hexaploidy population; 2x4x: diploid, tetraploid mixed population; 4x6x: diploid, tetraploid mixed population; 2x4x6x: diploid, tetraploid, hexaploid mixed population; unknown: ploidy level of plants in this population is unknown. The information on the ploidy level was extracted from Gross et al. (2025) PNAS, 122(33): e2505119122, https://doi.org/10.1073/pnas.2505119122. Ploidy_large: Indicating whether the major cytotypes in the population were diploid or polyploid. Diploid: only diploid Lithophragma bolanderi in the population; Polyploid: only tetraploid or hexaploid Lithophragma bolanderi in the population; Mixed: more than one major Lithophragma bolanderi cytotype in the population; Unknown: ploidy level of plants in this population is unknown. Ploidy_pop: Indicating whether one or more than one major Lithophragma bolanderi cytotype was present in the population. Pure: one major Lithophragma bolanderi cytotype was present in the population; Mixed: More than one major Lithophragma bolanderi cytotype was present in the population; Unknown: ploidy level of plants in this population is unknown. Lat: Latitude of the population in °N. Long: Longitude of the population in °E. Alt: Elevation of the population in m a.s.l. DWPOL_AcrossPops.txt: This tab-delimited text file contains data on the Greya politella offspring of one developing and one withering capsule for Litnophragma bolynderi plants from which we could collect a developing and a withering capsule. This data file was used in the R-script file Script_StatisticsForMS.R and contains the following columns: Pop: Three-letter abbreviation of the population name. PlantID: Individual letter code for each plant. DW: Indicating whether the capsule was developing (D) or withering (W; empty). POLTraces: Indication of the presence (1) or absence (0) of traces (eggs, larvae, or eaten seeds) of Greya politella. POL: Indication of whether the capsule contained traces (eggs, larvae, or eaten seeds) of Greya politella (Yes) or not (No). EatenSeeds: Number of partly eaten, developing ovules. Only eaten seeds with more than half the seed coat remaining were noted to avoid counting several smaller fragments originating from the same seed. N_Eggs: Number of Greya politella eggs. N_Larvae: Number of Greya politella larvae. Ploidy: Ploidy level of the major cytotypes in the population. 2x: diploid population; 4x: tetraploid population; 6x: hexaploidy population. The information on the ploidy level was extracted from Gross et al. (2025) PNAS, 122(33): e2505119122, https://doi.org/10.1073/pnas.2505119122. Ploidy_pop: Indicating whether one or more than one major Lithophragma bolanderi cytotype was present in the population. Pure: one major Lithophragma bolanderi cytotype was present in the population. Ploidy2: Indicating whether the major cytotypes in the population were diploid or polyploid. Diploid: only diploid Lithophragma bolanderi in the population; Polyploid: only tetraploid or hexaploid Lithophragma bolanderi in the population. FieldSurvey_MIN.txt: This tab-delimited text file contains fruit-set data in the diploid-tetraploid mixed Litnophragma bolynderi population MIN of plants of known ploidy level. This data file, or a subset thereof, was used in the R-script file Script_StatisticsForMS.R and contains the following columns: MINPlantID: Individual number for each plant. Population: Three-letter abbreviation of the population name. Lat: Latitude of the plant in °N. Long: Longitude of the plant in °E. Altitude_m: Elevation of the plant in m a.s.l. PloidyLevel: Ploidy level of the plant. This was identified by the Dutch company Plant Cytometry Service (https://plantcytometry.com). 2x: diploid; 3x: triploid; 4x: tetraploid. Developing: Number of developing capsules, considering capsules 2 to 6 counted from the bottom of the inflorescence. Withering: Number of withering (empty) capsules, considering capsules 2 to 6 counted from the bottom of the inflorescence. Eaten: Number of capsules eaten by herbivores, considering capsules 2 to 6 counted from the bottom of the inflorescence. Malformed: Number of malformed capsules, considering capsules 2 to 6 counted from the bottom of the inflorescence. Missing: Number of capsules that were missing (e.g. broken off), considering capsules 2 to 6 counted from the bottom of the inflorescence. Morphology: Number of capsules collected to measure flower morphology as part of another study, considering capsules 2 to 6 counted from the bottom of the inflorescence. Bud: Number of capsules in the bud stage and, thus, in a too early stage to be pollinated, considering capsules 2 to 6 counted from the bottom of the inflorescence. NoCapsule: Number of capsules that could not be considered (e.g. because there were fewer than 7 capsules produced by an individual), considering capsules 2 to 6 counted from the bottom of the inflorescence. No_CapsulesScored: Number of capsules that could be scored. This was calculated as Developing + Withering + Eaten + Malformed + Missing. No_DandW: Number of capsules that were either developing or withering. This was calculated as Developing + Withering. CapsuleData_MIN.txt: This tab-delimited text file contains seed-set data and data on the Greya politella offspring of developing capsules collected the diploid-tetraploid mixed Litnophragma bolynderi population MIN of plants of known ploidy level. This data file, or a subset thereof, was used in the R-script file Script_StatisticsForMS.R and contains the following columns: Population: Three-letter abbreviation of the population name. PlantID: Individual number for each individual. Ploidy: Ploidy level of the plant. This was identified by the Dutch company Plant Cytometry Service (https://plantcytometry.com). 2x: diploid; 4x: tetraploid. CapsuleDestiny: Indicating the destiny of the capsule. D: developing. CapsuleID: Plant-specific individual number for each capsule. POLTraces: Indication of whether the capsule contained traces (eggs, larvae, or eaten seeds) of Greya politella (Yes) or not (No). POL: Indication of the presence (1) or absence (0) of traces (eggs, larvae, or eaten seeds) of Greya politella. WholeSeeds: Number of intact developing ovules. UndevOvules: Number of undeveloped ovules. EatenSeeds: Number of partly eaten, developing ovules. Only eaten seeds with more than half the seed coat remaining were noted to avoid counting several smaller fragments originating from the same seed. N_Eggs: Number of Greya politella eggs. NA: No Greya politella eggs were present. N_Larvae: Number of Greya politella larvae. NA: No Greya politella larvae were present. N_Offspring: Number of Greya politella offspring. This was calculated as N_Eggs + N_Larvae. NA: No Greya politella offspring were present. OffspringStage: Indicating the stage of development of the Greya politella offspring in the capsule. Egg: only Greya politella eggs were present; Larvae: Greya politella larvae or Greya politella eggs and larvae were present; NA: no Greya politella offspring were present. N_EggsLarvae: Number of Greya politella eggs for capsules with only Greya politella eggs but no larvae and number of Greya politella larvae for capsules with Greya politella larvae or eggs and larvae. NA: no Greya politella offspring were present. DevOvules: Total number of developing ovules. This was calculated as Whole seeds + EatenSeeds. TotOvules: Total number of ovules. This was calculated as DevOvules + UndevOvules. PropDev: Proportion of developing ovules. This was calculated as DevOvules / TotOvules. DWPOL_MIN.txt: This tab-delimited text file contains data on the Greya politella offspring from capsules of Litnophragma bolynderi plants of the diploid-tetraploid mixed Litnophragma bolynderi population MIN for which we were able to collect developing and withering capsules from the same plant. This data file was used in the R-script file Script_StatisticsForMS.R and contains the following columns: Population: Three-letter abbreviation of the population name. PlantID: Individual number for each plant. PloidyLevel: Ploidy level of the plant. This was identified by the Dutch company Plant Cytometry Service (https://plantcytometry.com). 2x: diploid; 4x: tetraploid; NA: ploidy level not known. CapsuleDestiny: Indicating whether the capsule was developing (Developing) or withering (Withering; empty). CapsuleNumber: Plant-specific individual number for each capsule. POLTraces: Indication of whether the capsule contained traces (eggs, larvae, or eaten seeds) of Greya politella (Yes) or not (No). POL: Indication of the presence (1) or absence (0) of traces (eggs, larvae, or eaten seeds) of Greya politella. PopCoordinates.txt: This tab-delimited text file contains geographic details and information on the ploidy level of the 36 Lithophragma bolanderi populations included in the study. This data file was used in the R-script file Script_AcrossPopMap.R and contains the following columns: Population: Three-letter abbreviation of the population name. Ploidy: Ploidy level of the major cytotypes in the population. 2x: diploid population; 4x: tetraploid population; 6x: hexaploidy population; 2x_4x: diploid, tetraploid mixed population; 4x_6x: diploid, tetraploid mixed population; 2x_4x_6x: diploid, tetraploid, hexaploid mixed population; unknown: ploidy level of plants in this population is unknown. The information on the ploidy level was extracted from Gross et al. (2025) PNAS, 122(33): e2505119122, https://doi.org/10.1073/pnas.2505119122 for all populations except MIN. For MIN, this information was identified by the Dutch company Plant Cytometry Service (https://plantcytometry.com). PloidyGroup: Ploidy level of the major cytotype categories. 2x: pure diploid population; 4x: pure tetraploid population; 6x: pure hexaploidy population; mixed: population with more than one major cytotype; unknown: ploidy level of plants in this population is unknown. Latitude: Latitude of the population in °N. Longitude: Longitude of the population in °E. Elevation: Elevation of the population in m a.s.l. Col: Colors used in Figure 2A to visualize the categories in the column PloidyGroup. R-script files Script_StatisticsForMS.R: This R-script file contains all codes for all statistical analyses for study and to generate the estimates used to draw all figures except Figure 2A and Figure 2F. This was done based on the following data files: FieldSurvey_AcrossPops.txt, CapsuleData_AcrossPops.txt, DWPOL_AcrossPops.txt, FieldSurvey_MIN.txt, CapsuleData_MIN.txt, DWPOL_MIN.txt. Script_AcrossPopMap.R: This R-script file contains the codes to generate the raw version of Figure 2A. This was done based on the data files: PopCoordinates.txt. File with the raw versions of the figures Figures.xlsx: This Excel file contains the raw version for all figures for the main text and the Supplementary Information except for Figure 2A and Figure 2F. The values used to generate these figures were extracted from the output of the R-script file Script_StatisticsForMS.R as indicated within the R-script file. The sheets are labelled with the figure numbers they contain.