High-throughput mapping of 2'-O-methylation in Trypanosoma brucei poly(A) RNAs using RibOxi-seq

In this study we used RibOxi-seq to identify 2'-O-methylation (Nm) present on mRNAs in single nucleotide resolution. Our analysis identified hundreds of Nm sites in mRNAs, predominantly in CDS and 3' UTR. Overall design: Trypanosoma brucei procyclic form was used to extract total RNA. The RNA was then polyA enriched before going through RibOxi-seq for 2'-O-methylation profiling