Expression data changes induced by one round migration through 3 μm pores in T-ALL cells

One round migration through 3 μm pores promotes changes in transcription of T-ALL cells Jurkat cells were maintained in culture in RPMI 1640 medium with L-glutamine and 125 mM HEPES (Sigma Aldrich, St. Louis, MO, USA) and 10% fetal bovine serum, FBS (Sigma-Aldrich) and maintained in 5% CO2 and 37ºC. Jurkat cells were allowed to migrate across 3 μm transwell inserts for 24h. Control (non-migrated cells) and one-round migration (ORM) cells were collected from the bottom chamber of Transwell inserts (Corning Costar, 6.5 mm diameter, 3 μm pore size) after 24h. Cells were lysed and mRNA was isolated and hybridized on Affymetrix microarrays to compare expression profiles. We used 2 replicates of each sample.