Gene expression analysis of Saccharomyces cerevisiae subjected to sulfur starvation

To determine the genome-wide changes in gene expression associated with depletion of sulfur in S. cerevisiae, we performed transcriptional profiling using RNA-seq before and at three time points after tranfer in medium without sulfur. Genes downregulated and upregulated were identified by differential gene expression analysis of the RNA-seq data using DESeq2. Overall design: RNA-seq was performed on three replicates of yeasts collected before and 40, 80 and 120 min after transfer to sulfur-free medium.