Characteristics of HBxAg and miR-148a in tumor and nontumor compartments from HBV-infected HCC patients.

a. Sex 1 = male; 2 = female.b. Hepatitis b surface antigen (HBsAg) presence in serum: 0 = no, 1 = yes.c. NT = non-tumor; T = tumor.d. Intensity of HBxAg staining was evaluated as 0 (no signal) through 3 (intense signal).e. Tissue distribution of HBxAg: S = scattered, L = lobular, D = diffuse.f. Negative ΔΔCt value = elevated expression of miR-148a in tumor; positive ΔΔCt value = elevated expression of miR-148a in non-tumor liver.g. Fold change is calculated as 2ΔΔCt. The average fold change for elevated miR-148a was calculated by adding up the fold-change from each patient with a positive ΔΔCt value divided by the number of patients with a positive ΔΔCt value. The positive ΔΔCt values listed in this table are plotted in Figure 1 and represent elevated miR-148a levels in liver compared to tumor. Parallel calculations were performed to determine the average fold change among patients with negative ΔΔCt values, which are plotted in Figure 1 as elevated miR-148a levels in tumor compared to liver.