Raw data for the role of acetyl phosphate in bypassing the cell membrane electrical potential sensor LytS

Data 1Autophosphorylation of LytS.Quantification of phosphorylated LytS bands by NIH Image J. Average of two trials.
Data 2Phosphorylation of LytR by acetyl phosphate.Quantification of phosphorylated LytR bands by NIH ImageJ.
Data 3Phosphorylation of LytR-N by acetyl phosphate.Quantification of phosphorylated LytR-N bands by NIH ImageJ.
Data 4Quantification of LytR bands in native-PAGEs (Figure 6A and B) by NIH Image J.
LytR protein was phosphorylated by acetyl phosphate and its
dephosphorylation by LytS was monitored by native-PAGE, whereby the
phosphorylated dimer LytR becomes monomer after dephosphorylation. The
column “corrected” represents the data after correcting for background
signal at 0 min, and considering the band labeled “Monomer”, in the
absence of LytS and ATP, as 100% unphosphorylated LytR.

数据集1：LytS的自动磷酸化。通过NIH Image J对磷酸化LytS条带的量化。两次试验的平均值。
数据集2：由乙酰磷酸催化的LytR磷酸化。通过NIH ImageJ对磷酸化LytR条带的量化。
数据集3：由乙酰磷酸催化的LytR-N磷酸化。通过NIH ImageJ对磷酸化LytR-N条带的量化。
数据集4：通过NIH ImageJ对原位-PAGE中的LytR条带进行量化（图6A和B）。LytR蛋白被乙酰磷酸磷酸化，其去磷酸化过程通过LytS监控，其中磷酸化的二聚体LytR在去磷酸化后变为单体。‘校正’列表示在0分钟时对背景信号进行校正后的数据，并考虑标记为‘单体’的条带，在无LytS和ATP的情况下，作为100%未磷酸化的LytR。