Gene expression in ATII cells transduced with adenovirus expressing constitutively active mutant HIF1a

To determine HIF1a target genes in ATII cells, we transduced freshly isolated rat ATII cells with an adenoviral vector expressing either GFP or a constitutively active mutant HIF1a construct. After 24 hours, RNA was extracted and subjected to microarray.为鉴定ATII细胞（alveolar type II epithelial cells）中的缺氧诱导因子1α（hypoxia-inducible factor 1α，HIF1α）靶基因，我们使用分别表达绿色荧光蛋白（Green Fluorescent Protein，GFP）或组成型激活突变型HIF1α构建体的腺病毒载体，对新鲜分离的大鼠ATII细胞进行转导。转导处理24小时后，提取细胞总RNA并开展基因芯片（microarray）分析。